Real-time PCR has become a widely used technique for a variety of applications, such as gene expression, mutational analysis, and pathogen detection. Many biotechnology companies offer …

From Ct analysis of your real-time PCR gene expression experiments, to allele cluster analysis of Applied Biosystems™ TaqMan® SNP Assay data, to CNV copy number determination, to …

Aug 14, 2025 · Real-Time PCR is a technique used to monitor the progress of a PCR reaction in real-time. At the same time, a relatively small amount of PCR product (DNA, cDNA or RNA) …

Jul 2, 2025 · Analyzing RT-PCR data accurately is crucial for deriving meaningful biological insights. This article delves into the essential steps and considerations for analyzing RT-PCR …

In Sections 5–7, we present sample protocols and data that demonstrate the use of real-time PCR in specific applications, namely, gene expression analysis, allelic discrimination, and …

Jul 28, 2025 · Real-time PCR data, whether absolute copy numbers or relative fold changes, provides quantifiable insights into biological processes. Results are typically presented in …

Real-time PCR solutions, from sample preparation to data analysis For over a quarter century, we’ve provided you with reliable reagents, instruments, and software for all your real-time PCR …

Dec 2, 2025 · Learn the principles behind quantitative PCR, including reaction setup, amplification monitoring, and data interpretation for accurate gene expression analys...

real-time PCR include: PCR theoretically amplifies DNA exponentially, doubling the number of target molecules with ea. h amplification cycle. When it was first developed, scientists …

The internet is filled with different methods, procedures, and workflows that can overwhelm you during data analysis, especially if this is your first time with RT-qPCR. This article is going to …